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. 2020 Jan 20;27(7):2131–2142. doi: 10.1038/s41418-020-0490-7

Fig. 5. PHD3 controlled goblet cell generation independent of its hydroxylase activity.

Fig. 5

a CCD841 cells were transfected with Myc-PHD3 or Myc-PHD3(H196A). After 24 h, the cells were harvested for determination of ATOH1. b Overexpression of PHD3(H196A) reduced ubiquitination of ATOH1. 293T cells were transfected with Myc- PHD3(H196A) or Myc-PHD3(H196A) plus Flag-ATOH1 vectors in the presence of HA-Ub vector. After 24 h, the cells were treated with MG132 (10 μM) for 4 h, followed by immunoprecipitation. c Determination of the expression of ATOH1 and Muc2 in mice small intestine and colon epithelial cells. d PAS stain of intestinal epithelia from Phd3(H196A)IEC-KI mice and control littermates. Male mice at age of 5 weeks old (the body weight of the mice is within normal limits) were selected randomly (n = 3). The down panel shows the number of goblet cells from mice intestinal epithelia. e Stain of mice intestinal epithelia with Muc2 antibody. f A proposed model of PHD3 regulating the ATOH1 expression and goblet cell generation. ns no significance.