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. 2020 Jun 22;10:10101. doi: 10.1038/s41598-020-67148-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Histograms of intensity of 1610 cm⁻¹ (lignin/phenylpropanoids) band normalized to the intensity of 1382 cm⁻¹ band (CH₂ vibrations) in the Raman spectra collected from four trees (A–D) over a 6 month period. The observed intensities are compared to the intensity of 1610 cm⁻¹ band (with identical normalization) in the Raman spectra collected from GHH trees (greenhouse healthy). qPCR status of the plants is shown on the top panel. P-values obtained from two-sample t-tests are displayed between the compared groups.