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. 2020 Jun 22;10:10014. doi: 10.1038/s41598-020-66003-y

Table 2.

Daily Growth Rate (DGR %), Agar yield (%), Sulfate (%), 3,6-Anhydrogalactose (3,6-AG %), Proteins (%) and R-phycoerythrin (R-PE mg g−1fw) obtained from Gracilaria gracilis samples cultured at different depths in two sites (Bizerte Lagoon and Bizerte Bay, Northeast Tunisia) analyzed by ANOVA at 95% confidence level.

Factors SS df MS F p
DGR (%)
Intercept 154.07 1 154.07 1557.88 <0.05
Site 1.22 1 1.22 12.36 <0.05
Depth 7.85 14 0.56 5.67 <0.05
Site*Depth 10.24 14 0.73 7.39 <0.05
Error 5.93 60 0.10
Agar (%)
Intercept 40636.38 1 40636.38 6314.463 <0.05
Site 382.75 1 382.75 59.475 <0.05
Depth 110.93 14 7.92 1.231 >0.05
Site*Depth 58.34 14 4.17 0.648 >0.05
Error 386.13 60 6.44
Sulfate (%)
Intercept 4932.622 1 4932.622 3507.418 <0.05
Site 176.084 1 176.084 125.207 <0.05
Depth 54.125 14 3.866 2.749 <0.05
Site*Depth 74.098 14 5.293 3.763 <0.05
Error 84.380 60 1.406
3,6-AG(%)
Intercept 97159.40 1 97159.40 36228.44 <0.05
Site 6518.91 1 6518.91 2430.75 <0.05
Depth 853.10 14 60.94 22.72 <0.05
Site*Depth 1196.31 14 85.45 31.86 <0.05
Error 160.91 60 2.68
Proteins(%)
Intercept 41150.41 1 41150.41 979.3834 <0.05
Site 36.62 1 36.62 0.8715 >0.05
Depth 976.25 14 69.73 1.6596 >0.05
Site*Depth 661.06 14 47.22 1.1238 >0.05
Error 2521.00 60 42.02
R-PE(mgg−1fw)
Intercept 31.92 1 31.92 535.76 <0.05
Site 6.30 1 6.30 105.83 <0.05
Depth 1.15 14 0.08 1.38 >0.05
Site*Depth 0.23 14 0.02 0.27 >0.05
Error 3.57 60 0.06