Table 1.
Strains used in this work.
| Name | Relevant genotype | Source |
|---|---|---|
| K699 | ade2-1 trp1-1 can1-100 leu2-3,112 his3-11,15 ura3 MATa | K.Nasmyth |
| *BF264-15D | ade1 his2 leu2-3,112 trp1-1 ura3∆ns | B.Futcher73 |
| **EGY48 | ura3 his3 trp1 6xLexAop-LEU2 MATα | R.Brent |
| yRQ301 | CRIB-TdTomato-KANr MATa | This work |
| yRQ302 | alk1::NATr alk2::HIS3 CRIB-TdTomato-KANr MATa | This work |
| yRQ500 | CRIB-TdTomato-KANr [pGAL-CDC42] MATa | This work |
| yRQ501 | CRIB-TdTomato-KANr [pGAL-CDC42-G12V] MATa | This work |
| yRQ502 | alk1::NATr alk2::HIS3 CRIB-TdTomato-KANr [pGAL-CDC42] MATa | This work |
| yRQ503 | alk1::NATr alk2::HIS3 CRIB-TdTomato-KANr [pGAL-CDC42-G12V] MATa | This work |
| yRQ100 | [CDC24-GFP] MATa | This work |
| yRQ101 | alk1::NATr alk2::HIS3 [CDC24-GFP] MATa | This work |
| yRQ451 | [CDC24-mEOS] MATa | This work |
| yRQ452 | [CDC24-46A-mEOS] MATa | This work |
| yRQ453 | [CDC24-28D-mEOS] MATa | This work |
| yRQ454 | alk1::NATr alk2::HIS3 [CDC24-mEOS] MATa | This work |
| yRQ455 | alk1::NATr alk2::HIS3 [CDC24-46A-mEOS] MATa | This work |
| yRQ456 | alk1::NATr alk2::HIS3 [CDC24-28D-mEOS] MATa | This work |
| *yRQ505 | rsr1::HIS3 bem1::KANr [BEM1] [CDC24-HA-GFP] MATa | This work |
| *yRQ506 | rsr1::HIS3 bem1::KANr [bem1-8] [CDC24-HA-GFP] MATa | This work |
| yRQ366 | bcy1::KANr [CDC24-GFP] MATa | This work |
| yRQ367 | alk1::NATr alk2::HIS3 bcy1::KANr [CDC24-GFP] MATa | This work |
| yRQ368 | ras1::TRP1 ras2::HPHr bcy1::KANr [CDC24-GFP] MATa | This work |
| yRQ369 | alk1::NATr alk2::HIS3 ras1::TRP1 ras2::HPHr bcy1::KANr [CDC24-GFP] MATa | This work |
| yAN33 | alk1::NATr alk2::HIS3 MATa | This work |
| yRQ358 | ras1::TRP1 ras2::HPHr bcy1::KANr MATa | This work |
| yRQ359 | alk1::NATr alk2::HIS3 ras1::TRP1 ras2::HPHr bcy1::KANr MATa | This work |
| **yRQ479 | [pADH-LexA-CDC24] [pGAL1-B42AD-HA] [LexAop-LacZ] MATα | This work |
| **yRQ480 | [pADH-LexA] [pGAL1-B42AD-HA-RAS2] [LexAop-LacZ] MATα | This work |
| **yRQ481 | [pADH-LexA] [pGAL1-B42AD-HA-RAS2-G19V] [LexAop-LacZ] MATα | This work |
| **yRQ490 | [pADH-LexA] [pGAL1-B42AD-HA-RAS2-S24N] [LexAop-LacZ] MATα | This work |
| **yRQ483 | [pADH-LexA-CDC24] [pGAL1-B42AD-HA-RAS2] [LexAop-LacZ] MATα | This work |
| **yRQ484 | [pADH-LexA-CDC24] [pGAL1-B42AD-HA-RAS2-G19V] [LexAop-LacZ] MATα | This work |
| **yRQ491 | [pADH-LexA-CDC24] [pGAL1-B42AD-HA-RAS2-S24N] [LexAop-LacZ] MATα | This work |
| YRQ102 | ras2::TRP1 [CDC-24-GFP] MATa | This work |
| yRQ73 | [eEGFP-RBD3] MATa | This work |
| yRQ74 | alk1::NATr alk2::HIS3 [eGFP-RBD3] MATa | This work |
| yRQ442 | ras1::TRP1 ras2::HPHr bcy1::KANr [eGFP-RBD3] MATa | This work |
| yRQ472 | rsr1::KANr [eGFP-RBD3] MATa | This work |
| yRQ84 | [CDC25-GFP] MATa | This work |
| yRQ85 | alk1::NATr alk2::HIS3 [CDC25-GFP] MATa | This work |
| yRQ93 | ira1::LEU2 ira2::URA3 MATa | This work |
| yRQ95 | alk1::NATr alk2::HIS3 ira1::LEU2 ira2::URA3 MATa | This work |
| yRQ395 | [pGAL-GFP-RAS2] MATa | This work |
| yRQ396 | alk1::NATr alk2::HIS3 [pGAL-GFP-RAS2] MATa | This work |
| yRQ492 | sec6-4 [pGAL-GFP-RAS2] MATa | This work |
| yRQ493 | alk1::NATr alk2::HIS3 sec6-4 [pGAL-GFP-RAS2] MATa | This work |
| yRQ197 | [GFP-SNC1] MATa | This work |
| yRQ198 | alk1::NATr alk2::HIS3 [GFP-SNC1] MATa | This work |
| yRQ478 | mif2-3 [GFP-SNC1] MATa | This work |
| yRQ479 | alk1::NATr alk2::HIS3 mif2-3 [GFP-SNC1] MATa | This work |
| yRQ444 | ras1::TRP1 ras2::HPHr bcy1::KANr [GFP-SNC1] MATa | This work |
| yRQ445 | alk1::NATr alk2::HIS3 ras1::TRP1 ras2::HPHr bcy1::KANr [GFP-SNC1] MATa | This work |
| yRQ538 | Alk1KD::URA3 Alk2KD::URA3 [GFP-SNC1] MATa | This work |
All strains used are isogenic to W303 apart from those carrying deletions of RSR1 and BEM1, which are isogenic to BF264-15DU (marked with * in the table) and those used in two hybrid assays which are isogenic to EGY48 (marked with**).