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. 2020 Jan 20;27(7):2117–2130. doi: 10.1038/s41418-020-0489-0

Fig. 3. FLIPinBγ/DL co-treatment enhances caspase activity.

Fig. 3

a, b HeLa-CD95 cells were pretreated with 40 µM FLIPinBγ for 2 h and stimulated with CD95L (50 ng/mL) or TRAIL (100 ng/mL). Cells were stained with caspase-3/7 Green Apoptosis Assay Reagent. Caspase activity was visualized with live cell imaging. The fluorescence of caspase-3/7-positive cells was detected by the IncuCyte™ live cell analysis system (Essen Bioscience). In the upper part representative images after 2 h of CD95L stimulation are shown in a (bf brightfield, C = 50 ng/mL CD95L, F + C = 40 µM FLIPinBγ + 50 ng/mL CD95L). c HeLa-CD95 cells were pretreated with 20 µM FLIPinBγ for 2 h, followed by stimulation with CD95L (60 ng/mL) for 3 h. Total cellular lysates were analyzed by western blot using the indicated antibodies. One representative experiment out of three independent experiments is shown. Protein quantification was carried out using the normalization to actin.