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. 2020 Jan 28;27(7):2206–2216. doi: 10.1038/s41418-020-0496-1

Fig. 2. BAFF induces NF-κB-dependent p62 accumulation in CLL cells.

Fig. 2

a CLL cells were pretreated with 5 μM BMS-345541 for 1 hr followed by 3 hr incubation with 200 ng/ml BAFF. Nuclear phosphorylated p65 was analyzed by IB. b Relative amounts of phosphorylated p65 in CLL cells from (a). c IB and d densitometric analysis of p62 in whole lysates of CLL cells treated as in (a). n = 3. e IB analysis of lipidated LC3 (LC3-II) in CLL cells pretreated with 10 μM chloroquine (Chlorq) for 2 h and stimulated with BAFF for 3 and 6 hr. f Relative LC3-II amounts in CLL cells treated as in (e). Mean ± sem. n = 5. *p < 0.05; **p < 0.01; ***p < 0.005; ****p < 0.001.