Fig. 3. mTORC1 mediates γδ T cell IFN-γ production through the upregulation of Eomes expression.

a, b Raptor-f/f and Raptor KO mice were sacrificed and γδ T cells were analyzed for T-bet and Eomes expression in spleen ex vivo. Each symbol represents an individual mouse. Statistics of 4–5 mice for each group was shown in (a) and representative staining gated on γδ T cells was shown in (b). c–f Eomes-f/f and Eomes KO mice were analyzed for γδ T cell or CD27⁺ γδ T cell IL-17 and IFN-γ staining in spleen ex vivo. Each symbol represents an individual mouse. Statistics of 4 mice for each group was shown in (c, e) and representative plots for Eomes-f/f and Eomes KO γδ T cells was shown in (d, f). g–j T-bet-f/f and T-bet KO mice were analyzed for γδ T cell or CD27⁺ γδ T cell IL-17 and IFN-γ staining in spleen ex vivo. Each symbol represents an individual mouse. Statistics of 6 mice for each group in (g, i) and representative FACS plots were shown in (h, j). Data are means ± SEM. ns, not significant; *P < 0.05, **P < 0.01 and ***P < 0.001(two-tailed unpaired t test). Data are representative of at least two independent experiments. Numbers indicate percentage of cells in quadrants.