Fig. 6. mTORC1, but not mTORC2, is required for the cytotoxicity of γδ T cells and tumor immunity.
a γδ T cells isolated from Raptor-f/f or Raptor KO mice and expanded for 6 days (Effector cells). B16F0 target cells were labeled CFSEhi as target cells and same number of WT splenocytes were labeled CFSElo as internal cell number control. Different numbers of effector γδ T cells were mixed with the target cells. Cells were co-cultured for 12 h and remaining target cells were analyzed by FACS. Statistics of B16F0 cells percentages for triplicates was shown in (a) and representative plots were shown in (b). c, d B16F0 cells (3 × 105 cells) and expanded Raptor-f/f or Raptor KO γδ T cells (3 × 105 cells) were co-injected into flanks of TCR δ−/− mice. Raptor-f/f γδ T cells + B16F0 were injected into the left flank, and Raptor KO γδ T cells + B16F0 cells were injected into the right flank. Tumor growth for seven mice for each group was calculated and shown in (c). d Tumors for each group were isolated at day 18 post tumor injection were shown. Data are means ± SEM. ns, not significant; *P < 0.05, **P < 0.01 and ***P < 0.001(two-tailed unpaired t test). Data are representative of at least two independent experiments.