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. 2020 Jun 22;10:10052. doi: 10.1038/s41598-020-66903-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Left ventricular contractile function was similar in hearts from middle-aged and older mice, regardless of sex. (A) Examples of pressure recordings from Langendorff-perfused hearts isolated from middle aged and older male mice. Recordings are shown at condensed (left) and expanded (right) time scales. (B–D) Scatterplots demonstrate that LVDP, + dP/dt and −dP/dt were similar in hearts from middle-aged and older mice of both sexes. Data were analyzed by two-way ANOVA with age and sex as main factors (post-hoc test was Holm-Sidak). Samples were hearts from 11 middle-aged male mice, 14 older males, 15 middle-aged females and 11 older females, respectively.