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. 2020 Jun 22;10:10052. doi: 10.1038/s41598-020-66903-z

Figure 3.

Figure 3

Maximal actomyosin Mg-ATPase activity increased with age in hearts from females but not males. (A) Actomyosin Mg-ATPase activity increased as calcium concentrations increased to the same extent in myofilaments from middle-aged and older male hearts. (B) In females, actomyosin Mg-ATPase activity increased with age at almost all calcium concentrations tested. Values represent the mean ± SEM values. Data were analyzed with a two-way repeated measures ANOVA, with age as the main factor, calcium concentration as the repeated measure and pairwise multiple post-hoc comparisons with a Tukey test. The * symbol indicates a significant effect of age. Values were significant for p < 0.05. Samples were hearts from 5 middle-aged male mice, 6 older males, 5 middle-aged females and 5 older females, respectively.