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. 2020 Jun 22;10:10052. doi: 10.1038/s41598-020-66903-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The Hill coefficients for the actomyosin Mg ATPase activity versus calcium curves were graded by frailty in males only. (A,D) Maximal actomyosin Mg-ATPase activity was not related to frailty scores in either males or females. (B,E) EC₅₀ values also were not correlated with frailty index scores in either sex. (C,F) In contrast, Hill coefficients were graded by the level of frailty, but only in males. Data were fitted with a regression line. Correlation coefficients and p values are indicated on each panel and a line was drawn for statistically significant correlations (p < 0.05). Samples were hearts from 5 middle-aged male mice, 6 older males, 5 middle-aged females and 5 older females, respectively.