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. 2020 Jun 22;10:10052. doi: 10.1038/s41598-020-66903-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Data illustrating the protein phosphorylation of major myofilament proteins in hearts from middle-aged and older males and females of both sexes. Gels illustrate phosphorylation staining (ProQ Diamond) and total protein staining (Coomassie blue) for all samples quantified in this study. Individual samples in each group are indicated numerically at the top of each lane. Protein standards at 25 and 75 kDa are visible in the ProQ images (lane labelled “S”) and the molecular weight ladder is visible in the Coomassie images (lane labelled “L”). Actin (42 kDa) was used as a loading control and is shown with an arrow on each Coomassie image. (A,B) Myofilament proteins from middle-aged and older male ventricles were separated on SDS-PAGE and stained with Pro-Q (left) to compare phosphorylation. They were also stained with Coomassie blue (right) to illustrate protein load. (C,D) Myofilament proteins from middle-aged and older females separated by Pro-Q (left) and stained with Coomassie (right). Major myofilament proteins are indicated on the left side of each panel and molecular weight markers (kDa) are shown on the right. Myosin-binding protein C (MyBP-C), myosin light chain-1 (MLC-1). The gels have been cropped to the edges; the uncropped gels are included as Supplementary Information Files 1–3. Samples were hearts from 5 middle-aged male mice, 6 older males, 5 middle-aged females and 5 older females, respectively.