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. 2020 Jun 22;11:3158. doi: 10.1038/s41467-020-16926-x

Fig. 3. TADs contribute to compartmentalization of γH2Ax signaling domains.

Fig. 3

JuiceBox browser snapshots showing paired γH2Ax and interactome measurements across Tcrb (a) or Myc (b). Locations of genes, gene segments, and regulatory elements are shown at the top of each panel. RNP target locations and V.4C data extraction viewpoints are shown as lightning bolts. Tracks represent values for γH2Ax CR-seq (Red, representative of two independent replicates each, scaled min to max) or V.4C (Gray, n = 2 merged independent replicates, 0–25 contacts). Bottom: JuiceBox Hi-C plots after coverage normalization (n = 2, merged independent replicates each). G1-arrested Lig4−/− v-abl cells were used for Tcrb DSBs, while cycling Rag2−/− cells were used for targeting DSBs to Myc. Dashed lines represent selected TAD locations. The IGV tracks under the Hi-C data also show TADs, as well as their insulation scores, derived from 40 kb bins (blue lines) or 20 to 100 kb bins (gray lines).