TABLE 2.
Intraindividual M. leprae genomic differences.
| Samples | Genotype | Mutation and amino-acid change | Genomic region |
NS |
SSS |
Other genomes with similar position mutated (variant,% reads, genotype) | ||
| % reads | Aligned reads | % reads | Aligned reads | |||||
| RB073-RN084 | 1A | T1824441C;Gly56Asp | ml1512 | 17% | 24 | - | - | - |
| RB053-RN022 | 1D | G1823127A; Ser494Leu | ml1512 | 35% | 26 | - | - | Ml10-98 (Ser494Ala, 95%, 4N) |
| 1823613_1823614insC;Asp332fs | ml1512 | 20% | 21 | - | - | ARLP-23 (Asp332fs, 80%, 2E) | ||
| RB074-RN095 | 1B- Bangladesh | G1823098A; Leu504Phe | ml1512 | - | - | 92% | 92 | - |
| G660474C; Val252Leu | metK | 75% | 12 | 100% | 92 | - | ||
| C2116695A; Pro100Thr | ml1750 | 48% | 21 | - | - | SM1, (Pro100Ser, 100%, 4N/O) | ||
| A2116670G; Gln108Arg | ml1750 | 20% | 20 | - | - | LRC-1A (Gln108His, 100%,1A) Md0536 (Gln108Arg 90%, 1D-Mada) | ||
| C2116490T; Arg168His | ml1750 | 19% | 16 | - | - | Br14-3 (5 Arg168Cys, 1%,3I) Arg168His: Br2016-17 (22%, 3I); Comore-3 (36%; 1D-Mada); Ml9-81 (29%; 4N) | ||
| RB069-RN165 | 1B- Bangladesh | C95231T; Leu34Phe | glpQ | 29% | 76 | - | - | - |
| C2121552T; Val226Ile | ml1752 | 15% | 94 | - | - | - | ||
Genomic differences between M. leprae genomes obtained from slit skin smears (SSS; RB) and nasal swabs (NS; RN) of the same MB patient. The table shows the percentage of mutated non-duplicated reads and the total number of non-duplicated reads aligned at the position of the mutation for each sample type. Several positions were partially or totally mutated in other M. leprae strains from different genotypes. dup, duplication; *, stop; ins, insertion; del, deletion.