TABLE 3B.
Freeze drying of QC-LNP using mannitol at different concentrations.
| Cryoprotectant | Initial size | With freeze drying |
|||
| Concentration (%, w/w) | |||||
| Mannitol | 0 | 2.5 | 5 | 10 | |
| Size (nm) | 156.93 ± 09.78 | ND | 183.60 ± 6.4 | 163.20 ± 13.42 | 160.06 ± 14.21 |
| Ri | – | ND | 1.17 ± 0.10 | 1.04 ± 0.06 | 1.02 ± 0.08 |
| RS | – | * | ** | *** | *** |
Ri, redispersibility index; RS, reconstitution score, ***redispersible within 20 s with mere mixing, **redispersible within 1 min, ND-not determined due to incomplete redispersion of cake. *Reconstitution requires high shear vortexing for 2 min, but the cake was not completely redispersed. Values are presented as mean ± SD (n = 3).