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. 2020 Jun 16;8:461. doi: 10.3389/fcell.2020.00461

FIGURE 2.

FIGURE 2

The ncRNA from the DLK1-DIO3 locus are highly expressed in stromal cells and whole tissue compared to epithelial cells. qRT-PCR showing MEG3 expression is higher in breast stromal cells (fibroblasts) and whole tissue than in D492 (left). (LEP – luminal epithelial cells, MEP – myoepithelial cells, BRENCs – breast endothelial cells). Results shown as mean ± SD. One-way ANOVA with Dunnett’s multiple comparisons test was used to test significance: **p ≤ 0.01; ****p ≤ 0.0001; n = 3. Expression of representative miRNAs MIR127 and MIR411 at the DLK1-DIO3 locus is higher in breast fibroblast than in D492 (right). LEP – luminal epithelial cells, MEP - myoepithelial cells. Results shown as mean ± SD. One-way ANOVA with Dunnett’s multiple comparisons test was used to test significance: *p ≤ 0.5; n = 3.