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. 2020 Jun 16;14:461. doi: 10.3389/fnins.2020.00461

FIGURE 2.

FIGURE 2

Effect of Mfsd2a overexpression on learning and memory of rats. (A,B) Representative immunofluorescence images of the rat hippocampus at 14 days after viral transfection: (A) sham group; (B) 2VO + Mfsd2a AAV group: stereotaxic injection of the Mfsd2a adeno-associated virus (AAV-CMV-Mfsd2a-ZsGreen) targeting the rat CA1 region. The nuclei were labeled with DAPI (blue). (C) Western blot band of Mfsd2a in the hippocampus of rats at different time points after transfection with the AAVs. (D) Quantification of the relative expression of Mfsd2a. *P < 0.05, **P < 0.01, vs. the sham group; n = 6 per group. (E) Discrimination ratio scores of each group in the novel object recognition test. (F) The escape latency on days 1–5 in the Morris water maze. (G) Time spent in the target quadrant during the spatial probe test. *P < 0.05, **P < 0.01, vs. the sham group; #P < 0.05, ##P < 0.01, vs. the 2VO + control AAV group; n = 9 per group. Scale bar = 500 μm.