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. 2020 Jun 16;10:929. doi: 10.3389/fonc.2020.00929

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Copyright © 2020 Cheng, Cao, Wu, Xie, Li, Guan and Tan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MALDI-TOF-MS spectra of N-glycans. MCF10A, MCF7, MDA-MB-231, and SK-BR-3 cells were cultured in 10 cm dishes, and N-glycans from these cells were isolated as described as M&M. The lyophilized N-glycans were dissolved in methanol/water (1:1, v/v) solution, and an aliquot of the mixture with DHB solution was spotted on an MTP AnchorChip sample target and air-dried. MALDI-TOF-MS was performed in positive-ion mode. Experiments were performed in biological triplicate, and representative N-glycan spectra were shown. Peaks (signal-to-noise ratio >6) were selected for relative proportion analysis. Detailed structures were analyzed using GlycoWorkbench software. Proposed structures were indicated by m/z value.