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. 2020 Jun 16;10:929. doi: 10.3389/fonc.2020.00929

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Copyright © 2020 Cheng, Cao, Wu, Xie, Li, Guan and Tan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Levels of bisecting GlcNAc on target glycoproteins. (A) Altered expression of EGFR and integrin β4 bearing bisecting GlcNAc. Antibody against EGFR and integrin (green) β4, PHA-E lectin (red), and DAPI (blue) were applied, and immunostaining was performed as described as M&M. Fluorescence signals were photographed using confocal microscope. (B) Expression of target glycoproteins with bisecting GlcNAc by immunoprecipitation. MCF10A and MDA-MB-231 cell lysate were subjected to immunoprecipitation with antibody against EGFR (B), and immunoprecipitates were subjected to immunoblot analysis. **p < 0.01.