Figure 6.

S100A8 and S100A9 proteins in cord blood plasma in preterm and term newborns and in relation to S100A gene expression and clinical features associated with chorioamnionitis and fetal inflammation within the preterm group. Plasma protein levels of S1000A8 and S100A9 were analyzed by mass-spectrometry and relative concentrations were compared between groups. The first panel shows that term infants (n = 10) had significantly higher levels than preterm infants (n = 33). The remaining panels all refer to differences in plasma protein levels within the preterm group. Elevated plasma protein levels were seen in infants with high (n = 17) compared with low (n = 16) S100A8 and S100A9 gene expression. Preterm labor (PTL)/preterm prelabor rupture of membranes (PPROM), histological chorioamnionitis (HCA), and histological fetal inflammatory response syndrome (FIRS) were also associated with increased protein levels, while exposure to elevated CRP/IL-6 in cord blood plasma was not (individual samples demonstrated in figure). Data presented as mean ± SEM and compared with unpaired t-test for log2-transformed relative concentrations of S100A8 and S100A9 proteins, p-values in figure.