Figure 5.

Frequencies of IETs expressing CD27 decreased with age in distal intestinal tract. (A) Comparisons of percentages of CD27⁺ cells within CD2⁺CD8α⁺ γδ IETs across timepoints within a single intestinal tissue. (B,C) Fluorescence intensities of CD27 from total CD2⁺CD8α⁺ γδ IETs within a single intestinal tissue across time (B) or within a single timepoint across intestinal tissues (C). (D) Comparisons of percentages of CD27⁺ cells within CD4⁻CD8α⁺ αβ IETs across timepoints within a single intestinal tissue. (E,F) Fluorescence intensities of CD27 from CD4⁻CD8α⁺ αβ IETs within a single intestinal tissue across time (E) or within a single timepoint across intestinal tissues (F). In (B,C) and (D,E), frequency is proportional to unit area under the curve, and all samples are represented by equal total unit area under the curve. For fluorescence intensity curves, data are from merging the subsetted data of 8 samples from each timepoint/tissue combination used for t-SNE analysis. The same data are presented in B and C or D and E to show age-dependent (B,D) or tissue-dependent (C,E) changes. Statistical significance was determined for percentages of total CD27⁺ cells within a single tissue across timepoints by the rank-based Kruskal-Wallis test using all combinations of multiple comparisons within a tissue. P < 0.05 were considered significant (* <0.05, ** <0.01, *** <0.001). Samples were taken from four animals per timepoint in each of two separate experiments (n = 8 per timepoint; n = 24 total).