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. 2020 Jun 16;11:579. doi: 10.3389/fphys.2020.00579

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Copyright © 2020 Hosotani, Kawasaki, Hasegawa, Wakasa, Hoshino, Takahashi, Ueda, Takaya, Iwasaki and Watanabe.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Histology of the pectoralis major muscle of broilers stained with Azan and hematoxylin–eosin. (A–E) Histological observations of the pectoralis major muscle of (A) sample number 1 (stage I; FA, 7.24% and CM, 0.70), (B) 2 (stage I; FA, 9.06% and CM, 0.69), (C) 3 (stage I; FA, 13.25% and CM, 0.68), (D) 33 (stage VI; FA, 40.90% and CM, 0.70), and (E) 34 (stage VI; FA, 40.97% and CM, 0.76). The squares indicated by dashed lines are magnified on the right. White bar = 200 mm, black bar = 100 mm. SV, myofiber with numerous small vacuoles; RV, myofiber with large-rimmed vacuoles; H, hypertrophy of myofiber; SC, myofiber with small caliber; SF, split fiber; D, myofiber degeneration; FA, fibrotic area in the muscle; CM, circularity of myofibers.