TABLE 1.
Gene | Accession number | Primer sequence (5′–3′) F: Forward, R: Reverse | Product size (bp) |
ACTB | NM_205518.1 | F: ACAATGGCTCCGGTATGTG | 133 |
R: CCAACGTAGCTGTCTTTCTGG | |||
DRP1 | NM_001079722.1 | F: GGTGATCAACAAGCTGCAAGA | 180 |
R: GCACCAGCTGCAGAATAAGG | |||
HIF1A | NM_204297.1 | F: GTCACCGACAAGAAGAGGATTAG | 225 |
R: TTCTGTCTCTAGCTCACCAGCA | |||
MAP1LC3A | XM_417327.6 | F: GTACAGCAGATCCGAGAGCA | 298 |
R: AGGTCTCCTGGGAAGCGTAG | |||
MAP1LC3B | NM_001031461.1 | F: ACGTCAACATGAGCGAGCTA | 197 |
R: TTGCACTCCGAAAGTCTCCT | |||
MFN1 | NM_001012931.2 | F: TGGGATTGGCCATACAACTAAC | 277 |
R: GAATTTGTCAATCCAGCTGTCC | |||
MFN2 | XM_015297205.2 | F: TGATGGGCTATAACGACCAGA | 208 |
R: GAGAGAGCAATCAGCCTCCA | |||
OPA1 | NM_001039309.1 | F: TGATTGCTCAAGCCCGAATA | 250 |
R: CCAGGACCTCTCACACTTAAGGA | |||
PARK2 | XM_419615.6 | F: GGAGAAGAACAGTACAACCGCTAC | 182 |
R: CATTCACGGCAGAACACAA | |||
VEGFA | NM_205042.2 | F: ACAATTGAGACCCTGGTGGA | 195 |
R: CGCTATGTGCTGACTCTGATG |
ACTB, actin, beta; DRP1, dynamin-related protein 1; HIF1A, hypoxia inducible factor 1 subunit alpha; MAP1LC3A, B, microtubule associated protein 1 light chain 3 alpha, beta; MFN1, 2, mitofusion 1, 2; OPA1, OPA1, mitochondrial dynamin like GTPase; PARK2, parkin RBR E3 ubiquitin protein ligase; VEGFA, vascular endothelial growth factor A.