TABLE 1.

Functions of miRNAs targeting Timp3 and their binding sites in 3′ UTR of Timp3 mRNA.

miRNA	Cell/tissue	Binding site	Function	References
miR-17	Cardiomyocyte, H9C2, 293T	1351−1357	Hypertrophy↑, Proliferation↑, survival↑; TIMP3 ↓	Shi et al., 2017; Liu et al., 2018
	Prostate cancer cells, Bladder cancer cells	101−123, 524−545	Proliferation ↑, Survival ↑, migration ↑, invasion ↑, colony formation ↑, tumorigenesis ↑; TIMP3 ↓	Yang X. et al., 2013; Peng and Li, 2019
miR-21	HUVECs	1032−1039	Survival ↑, migration ↑, capillary formation ↑; TIMP3 ↓, MMP2 ↑, MMP9 ↑	Hu et al., 2016
	RCC cell, 293T,cervical cancer cell	1032−1039	Proliferation ↑, migration ↑, invasion ↑; TIMP3 ↓	Chen et al., 2017; Zhang Z. et al., 2018
	Podocytes, 293T	2322−2329	Apoptosis ↑, IL-1β↑, TNF-α↑, Bax ↑; TIMP3 ↓	Chen X. et al., 2018
miR-34b	MRC-5, 293T	1282−1288	Fibrosis ↑, col I ↑; TIMP3 ↓	Hu et al., 2019
miR-136	Neuronal cell	2582−2600	Apoptosis ↓; TIMP3 ↓	Jin et al., 2017
miR-142	DRG neuron cells, 293T	55−61	Viability ↑, apoptosis ↓; TIMP3 ↓	Wu et al., 2018
miR-181b	Cardiomyocytes, 293T	797−811	Apoptosis ↓; TIMP3 ↓	Gao et al., 2017
	HCC	3499−3505, 3573−3580	Growth ↑, clonogenic survival ↑, migration ↑, invasion ↑, tumorigenicity ↑; TIMP3 ↓, MMP2 ↑, MMP9 ↑	Wang et al., 2010
miR-191	Prostate cancer cell	3553−3573	Growth ↑, invasion ↑; TIMP3 ↓	Wang X. et al., 2018
miR-205 (miR-712)	iMAECs, VSMCs	3495−3500	AAA ↑, inflammation ↑, atherosclerosis ↑, VSMC migration ↑, EC permeability ↑; TIMP3 ↓, RECK ↓, MMP activity ↑, ADAM activity ↑, ADAMTS4 activity ↑	Son et al., 2013; Kim et al., 2014
miR-206	THP-1, CFb, 293T	1135−1146, 1683−1689	Inflammatory cytokines ↑; TIMP3 ↓, MMP2 ↑, MMP9 ↑	Limana et al., 2011; Fu et al., 2016
miR-221	PTC cell, 293T	2429−2450	Proliferation ↑, apoptosis ↓, migration ↑; TIMP3 ↓	Diao et al., 2017
miR-222	PASMCs, NSCLC, HCC, OS	2443−2449	Migration ↑, proliferation ↑, invasion ↑; TIMP3 ↓	Garofalo et al., 2009; Xu et al., 2017; Guo et al., 2018
miR-323a	CFb, 293T	3590−3596	Proliferation ↑, fibrosis ↑, Col I ↑, col III ↑, TGF-β↑; MMP2 ↑, MMP9 ↑, TIMP3 ↓	Zhang J. et al., 2018
miR-365	rMC-1, 293T	336−342	Gliosis ↑, oxidative stress ↑; TIMP3 ↓	Wang J. et al., 2018
miR-373	ESCC cell lines	112−118	Migration ↑, proliferation ↑, invasion ↑; TIMP3 ↓	Liu et al., 2016
miR-770	Podocytes	227−232	Apoptosis ↑, IL-1β↑, TNF-α↑; TIMP3 ↓	Wang and Li, 2020

293T, human embryonic kidney 293T cell; AAA, abdominal aortic aneurysm; CFb, cardiac fibroblasts; col, collagen; DRG, dorsal root ganglion; ESCC, Esophageal squamous cell carcinoma; H9C2, rat heart myoblast H9C2 cells; HCC, hepatocellular carcinoma cells; HUVECs, human umbilical vein endothelial cells; iMAECs, immortalized mouse aortic endothelial cells; MRC5, human lung fibroblast line; NSCLC, non-small cell lung cancer cells; OS, osteosarcoma cell; PASMCs, pulmonary artery smooth muscle cells; PTC, papillary thyroid cancer; RCC cell, renal cell cancer cell; RECK, reversion-inducing cysteine-rich protein with kazal motifs; rMC-1, rat Müller cell line; THP-1, human THP-1 macrophages; VSMCs, vascular smooth muscle cells. ↑, increased or activated; ↓, decreased or inactivated.