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. 2020 Jun 16;11:823. doi: 10.3389/fpls.2020.00823

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Copyright © 2020 Llebrés, Pascual, Valle, de la Torre, Valderrama-Martin, Gómez, Avila and Cánovas.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Subcellular localization of PpPIIa and PpPIIb. (A) Localization in N. benthamiana leaves. (A1,A2) GFP-chlorophyll merged images showing respective localizations in the chloroplast of PpPIIa and PpPIIb. (B) Localization in isolated pine protoplasts. A single cell is shown containing plastids. (B1) control with no construct; (B2) GFP–PpPIIa construct containing transit peptide (cTP); (B3) GFP-PpPIIa construct without cTP; (B4) GFP-control construct without the PpPIIa protein but containing cTP. Scale bar represents 10 μm.