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Estimation of the molecular size of native PpPII proteins by gel filtration chromatography. (A) Elution profile of recombinant PpPIIa and PpPIIb proteins using gel filtration in an FPLC system. (B) Protein-containing fractions were tested by western blotting using monospecific antibodies against PpPIIa. (C) Estimation of PpPIIa and PpPIIb molecular mass using protein markers of known molecular size.
