Figure 5. High throughput detection of Plasmodium falciparum signal from pooled excreta/feces (E/F).

Individual Anopheles gambiae mosquitoes were exposed to P. falciparum trophozoites at a parasitemia of 0.1%. E/F was then collected from mosquitoes, either individually, or following pooling with 49 additional A. gambiae mosquitoes having been exposed to a parasite-naïve bloodmeal. All E/F samples were then tested by qPCR, in triplicate reactions, for the presence of P. falciparum signal. Mean results from the testing of each pool are indicated. Significance, as determined by the results of unpaired t tests, is provided. ** p < 0.01.