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. 2020 Jun 3;3:1734. Originally published 2019 Dec 19. [Version 2] doi: 10.12688/gatesopenres.13093.2

Figure 3. Detection windows for parasites in the excreta/feces (E/F) of mosquitoes following a Plasmodium falciparum-containing bloodmeal.

Figure 3.

( A) Individual Anopheles gambiae mosquitoes were exposed to a P. falciparum-containing bloodmeal at a parasitemia of 5000 trophozoites/mL (0.1% parasitemia), 500 trophozoites/mL (0.01% parasitemia) or 50 trophozoites/mL (0.001% parasitemia). E/F was then collected from each mosquito, individually, in 24 hour time blocks. Collected E/F samples were then tested in triplicate qPCR reactions. Colors represent Cq values, and numbers in parenthesis indicate the number of positive qPCR replicates. ( B) A comparison of the mean Cq values from all qPCR-positive E/F samples (irrespective of time point) produced by mosquitoes at each tested parasitemia. Significance, as determined by the results of unpaired t tests, is provided. ** p < 0.01, *** p < 0.001. ( C) Testing, in duplicate dPCR reactions, of the same samples collected in panel A. Colors represent the number of positive sample wells per reaction, and numbers in parenthesis indicate the number of positive dPCR replicates. ( D) A comparison of E/F sample positivity as determined by qPCR and dPCR results from panels A and C.