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. 2020 Jun 16;11:677. doi: 10.3389/fphys.2020.00677

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Copyright © 2020 Lai, Fealy, Kummitha, Cabras, Kirwan and Hoppel.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Respiration rates in permeabilized soleus (A) and white gastrocnemius (B) muscles of control and GK rats obtained under leak state (P_L,N) and oxphos state (P_P) with malate and pyruvate, cytochrome c (Cyt-c), Succinate (S_P), uncoupled mitochondria to measure maximal electron transport chain capacity (ET), rotenone (R). Also, ET-R indicates the respiration rate difference between that indicated with ET and R; CIV indicates the respiration rate difference between that with ascorbate plus TMPD and azide; Respiratory acceptor control ratio (RACR). Data are mean ± SEM (n = 6). For soleus, the respiration rate obtained with cytochromes c is 8 ± 3% and 14 ± 7% greater than that in presence of only P for control and GK, respectively. And for white gastrocnemius is 5 ± 4% and 2 ± 2%, respectively.