TABLE 2.

Comparison of MSC culture components used by each of the five sites.

Components	Site #1	Site #2	Site #3	Site #4	Site #5
Cells plated	MNC	Whole BM	Whole BM	MNC	Whole BM or MNC
Basal medium	αMEM	αMEM	αMEM	MEM-Eagle	DMEM
Serum supplement	16% FBS	20% FBS	10% FBS	16.5% FBS	10% PL-S
Additional supplements	GlutaMAX	None	L-glutamine	L-glutamine	GlutaMAX
Antibiotics	None	Gentamicin	Pen/Strep	Pen/Strep	Pen/Strep

MNC, ficoll preparation; aMEM, minimum essential medium eagle alpha modification; DMEM, dulbecco’s modified eagle medium; FBS, fetal bovine serum; PL-S, serum based platelet lysate; glutaMAX an alternative to L-glutamine; Pen/Strep, penicillin streptomycin.