TABLE 3.

Plating densities for each marrow preparation by site and passage.

Culture vessel type and passage number	Number of cells plated/cm2 (Average ± SD)
	*Site #1	*Site #2	*Site #3	*Site #4	*Site #5
Culture vessel type	Cell stack	Flasks	Flasks	15 cm dishes	Flasks
Initial seeding to obtain P0 MSCs	136,667 ± 23,094	282,000 ± 5,292	200,407 ± 43,808	19,737 ± 0	240,478 ± 38,708
Second seeding of P0 MSCs to obtain PI MSCs	50 ± 1	3,165 ± 1	2,500 ± 707	2,758 ± 4,425	2,776 ± 1,807
Third seeding of P1 MSCs to obtain P2 MSCs		3,165 ± 1	*4,055		**2,336 ± 232
Fourth seeding of P2 MSCs to obtain P3 MSCs		3,164 ± 1			***2,000

*Average ± Std. Dev. n = 3; **n = 2; ***n = 1.