TABLE 4.

Cryopreservations methods used by each site.

	Site #1	Site #2	Site #3	Site #4	Site #55
Cell concentration	1–10 × 106 Cells/mL	1–10 × 106 Cells/mL	1.5–5 × 106 Cells/mL	5 × 106 Cells/mL	1–5 × 106 Cells/mL
Final Freezing Solution	30% Plasmalyte-A, 10% DMSO, 2.5% Human serum albumin	Plasmalyte A, 6% Pentastarch, 5% DMSO, 2%, Human serum albumin	60% D-MEM, 30% Hyclone FBS, 10% DMSO	MEM-Eagle 15% FBS, 10% DMSO	90% FBS, 10% DMSO
Freezing Method	Control Rate Freezer	Mr. Frosty Freezing Container	Mr. Frosty Freezing Container	No Container	Mr. Frosty Freezing Container
	Start Chamber = 0.0°C;	−80°C O/N; transfer	−80°C O/N; transfer	−80°C O/N; maintain	−80°C O/N; transfer
	Sample = 1.0°C	to LN2 freezer	to LN2 freezer	in −80°C freezer	to LN2 freezer
	Ramp 1.0°C/min until
	Sample = –12.0°C
	Ramp 20.0°C/min until
	Chamber = –60.0°C
	Ramp 15.0°C/min until
	Chamber = –18.0°C
	Ramp 1.0°C/min until
	Sample = –60.0°C
	Ramp 3.0°C/min until
	Sample = –100.0°C
	End and transfer to LN2
	Freezer
Storage freezer	LN2	LN2	LN2	−80°C	LN2