FIGURE 3.

Deletion of HXK1 causes a decreased expression of HXK2 and GLK1/4. Cells were grown at 30°C in SC medium supplemented with a specific carbon source. RT-qPCR was used to study gene expression. (A) Expression levels of the different genes when cells were grown on SC with 2% glucose. (B) Expression levels of the different genes when cells were grown on SC with 2% fructose. (C) Expression levels of the different genes when cells were grown on SC with 2% glycerol. The average of two independent experiments is shown and the error bars represent the SEM. Statistical analysis was done on log₂(y)-transformed values by using an ANOVA test with Bonferroni correction. For the expression of HXK1 and GLK1/4 on fructose, a t-test was used. A significant difference was found when *p = 0.0332, **p = 0.0021, ***p = 0.0002 or ****p < 0.0001.