TABLE 2.

Composition (in relative abundance) of sterols of the mutant and parental strains of X. dendrorhous analyzed by HPLC/MS.

	Strain
Metabolite	CBS 6938	CBS.Δerg3	CBS.Δerg4	CBS.cyp61–
Ergosterol	36,000 ± 2,160	ND	ND	ND
Ergosta-5,7,22,24(28)-tetraenol	700 ± 42	ND	14,000 ± 980	ND
Ergosta-5,7,24(28)-trienol	800 ± 56	ND	3,200 ± 192	20,000 ± 1,200
Episterol	10,000 ± 600	26,000 ± 1,820	24,000 ± 1,440	18,000 ± 900
Fecosterol	3,000 ± 180	5,300 ± 371	6,300 ± 330	3,000 ± 180
Zymosterol	3,500 ± 210	3,500 ± 210	2,500 ± 150	3,000 ± 180
Lanosterol	3,200 ± 192	14,000 ± 980	14,000 ± 980	13,000 ± 910

Table shows the mean of the relative abundance of the majority ion of each molecule ± standard deviation of three independent cultures of each strain. ND, not detected.