Fig. 1. A chemical genetic cell-based screen to identify compounds targeting SALL4 dependencies.

(A) Schematic of screen involving the use of endogenous SALL4^lo and SALL4^hi HCC lines and engineered isogenic SALL4 expressing lines. (B) Venn diagram illustrating overlap of hit compounds which selectively decrease cell viability of the SALL4^hi lines over their respective SALL4^lo controls. (C) Workflow of natural product extract screen to identify individual compound hits from extracts containing multiple chemical entities.