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. 2020 Apr 16;15(6):1613–1620. doi: 10.1021/acschembio.0c00208

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Copyright © 2020 American Chemical Society

This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes.

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In vivo homing assay confirmation of no functional differences in splenocytes labeled with DACT-1 compared to splenocytes labeled with eFluor670. (A) Representative FACS analysis of eFluor670⁺DACT-1^– and eFluor670⁺DACT-1⁺ splenocytes (1:1 ratio). (B) Representative FACS analysis of LN cell suspensions showing percentages of labeled cells. (C) Analysis of ratios of DACT-1⁺ to DACT-1^– cells in LNs; values were determined using the gating strategies displayed in B. Pooled data from three independent experiments are displayed. Results were normalized to the input and displayed as individual data points (n = 3) and mean (line).