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. 2020 Apr 27;15(6):1306–1312. doi: 10.1021/acschembio.0c00285

Figure 3.

Figure 3

(A) HER2+ SK-BR-3 cells were treated with Alexa488-conjugated Ab-PROTAC (AF488-3) (100 nM) for 1, 4, 8, and 24 h (all post-1-h washout). Cells were then labeled with LysoTracker DeepRed (50 nM, 30 min). Shown is a representative frame from live imaging of cells via confocal microscopy. Scale bar = 20 μm. (B) HER2– MCF-7 cells were treated with AF488-3 (100 nM) for 30 min, followed by incubation with LysoTracker DeepRed (50 nM) for an additional 30 min. Shown is a representative frame from live imaging of cells via confocal microscopy. (C) Quantification of AF488-3 colocalization with LysoTracker DeepRed. The Pearson’s correlation coefficient is shown for various incubation time points in SK-BR-3 cells; data are mean + the standard error of measurement (SEM); n = 6 cells. Statistical analysis was performed by one-way ANOVA, followed by Tukey’s multiple comparison test (triple asterisk symbol (***) denotes P < 0.0001; “ns” = nonsignificant).