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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: Biochem Pharmacol. 2019 Nov 21;171:113728. doi: 10.1016/j.bcp.2019.113728

Figure 3: X1 induces JNK activation.

Figure 3:

Cells in whole media were treated with erastin (10 μM) for 3 h (A, HepG2 cells) or X1 for 2 h (10 μM) (B, Huh7 cells). Proteins were extracted at progressive time points, and Western blotting for p-JNK were performed, as described in MATERIAL &METHODS. In (C), Huh7 cells were treated with X1 (10 μM) for 30 min with and without pretreatment with SP600125 (30 μM) for 1 h or NAC (100 μM) for 30 min before immunoblotting for pJNK, total JNK and β-actin as a loading control. In (D), pJNK/JNK was determined by densitometry and plotted. *p<0.05 vs vehicle, #p<0.05 vs X1 from 3 independent experiments. SP: SP600125; NAC: N-acetyl cysteine.