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The role of RSK3 in regulating RGC‐5 necroptosis. (a) Annexin V/PI staining of RGC‐5 detected by flow cytometry. (b) The statistical analysis of flow cytometry results. **vs. OGD 4 hr/R 4 hr, p < .01. (c) The percentage of RGC‐5 necrosis was determined by LDH release assay following LJH685+OGD. **vs. OGD 4 hr/R 4 hr, p < .01. (d) The percentage of RGC‐5 necrosis was determined by LDH release following rsk3‐siRNA+OGD. ***vs. OGD 4 hr/R 4 hr, p < .001
