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(a) MALDI-TOF MS data of CS5 before (blue) and after (red) treatment with CDAP and hypothetical structure of CS5. (b) SDS-Tricine gel of CS5 coexpressed with NisBTC or NisT before (&) and after (#) streptavidin column purification. (c) LC-MS/MS data of CS5(13–20). The observed fragments demonstrate that the cyclic strep ligand was formed.
