Table 1. Dehydration of Designed Peptides by Different NisB Mutationsa.
| observed
mass (Da, main product)/dehydrations |
|||||
|---|---|---|---|---|---|
| peptide | predicted mass (Da)/dehydrations | NisBwt | NisBmut(S88P/D234N) | NisBmut(S88P) | NisBmut(D234N) |
| nisin | 5688/8 | 5689/8 | 5687/8 | 5687/8 | 5687/8 |
| CS5D | 5477/5 | 5496/4 | 5477/5 | 5495/4 | 5495/4 |
| CS5A | 5433/5 | 5434/5 | 5434/5 | 5432/5 | 5433/5 |
| nisinT2D | 5720/7 | 5739/6 | 5723/7 | 5736/6 | 5737/6 |
| nisinT2E | 5734/7 | 5747/6 | 5732/7 | 5734/7 | 5732/7 |
| nisin(1–22)K12D | 4460/5 | 4479/4 | 4479/4 | 4477/4 | 4476/4 |
| nisin(1–22)K12E | 4474/5 | 4473/5 | 4473/5 | 4472/5 | 4471/5 |
| nisin(1–22)G14D | 4531/5 | 4548/4 | 4532/5 | 4531/5 | 4530/5 |
| nisin(1–22)G14E | 4545/5 | 4559/4 | 4542/5 | 4543/5 | 4543/5 |
a
NisinT2D, the second amino acid of nisin was changed from Thr to Asp; nisinT2E, the second amino acid of nisin was changed from Thr to Glu; nisin(1–22)K12D, the 12th amino acid of nisin was changed from Lys to Asp; nisin(1–22)K12E, the 12th amino acid of nisin was changed from Lys to Glu; nisin(1–22)G14D, the 14th amino acid of nisin was changed from Gly to Asp; nisin(1–22)G14E, the 14th amino acid of nisin was changed from Gly to Glu.
b
The major products with less dehydration than predicted are in italic. The major products with predicted dehydration are in bold.