| Strains Characterized in This
Study |
| IMX1770 |
MATaura3–52his3–1leu2–3,112MAL2–8cSUC2hxk1::KlLEU2tdh1tdh2gpm2gpm3eno1pyk2pdc5pdc6adh2adh5adh4pyk1pgi1tpi1tdh3pfk2::(pTEF-cas9-tCYC1
natNT1)pgk1gpm1fba1hxk2pfk1adh1pdc1eno2glk1::Sphis5Δ::(pGAL1-I-SceI-tCYC1)can1::(ARS418CAN1 AHFBA1_*AH HTPI1_*HPPGK1_*PQADH1_*QNPYK1_*NOTDH3_*OAENO2_*ABHXK2_*BCPGI1_*CDPFK1_*DJPFK2_*J BPHIS3BP LGPM1_*LMPDC1_*M ARARS1211AR CAN1)
sga1::KlURA3
|
This study; Prototrophic strain
with watermarked single locus
glycolysis (WMG strain); Derived from IMX1717 |
| IMX1771 |
MATaura3–52his3–1leu2–3,112MAL2–8cSUC2hxk1::KlLEU2tdh1tdh2gpm2gpm3eno1pyk2pdc5pdc6adh2adh5adh4pyk1pgi1tpi1tdh3pfk2::(pTEF-cas9-tCYC1 natNT1)pgk1gpm1fba1hxk2pfk1adh1pdc1eno2glk1::Sphis5Δ::(pGAL1-I-SceI-tCYC1)can1::(ARS418CAN1 AHFBA1AH HTPI1HPPGK1PQADH1QNPYK1NOTDH3OAENO2ABHXK2BCPGI1CDPFK1DJPFK2J BPHIS3BP LGPM1LMPDC1M ARARS1211AR CAN1)sga1::KlURA3
|
This study; Prototrophic strain with native single
locus glycolysis
(NG strain); Derived from IMX1747 |
| IMX2028 |
MATa ura3–52his3–1leu2–3,112MAL2–8cSUC2hxk1::KlLEU2tdh1tdh2gpm2gpm3eno1pyk2pdc5pdc6adh2adh5adh4glk1::Sphis5Δ::(pGAL1-I SceI-tCYC1)can1::(ARS418CAN1 AHFBA1AH HTPI1HPPGK1PQADH1QNPYK1NOTDH3OAENO2ABHXK2BCPGI1CDPFK1DJPFK2J BPHIS3BP LGPM1LMPDC1M ARARS1211AR CAN1)sga1::(ARS418sga1 AHFBA1_*AH HTPI1_*HPPGK1_*PQADH1_*QNPYK1_*NOTDH3_*OAENO2_*ABHXK2_*BCPGI1_*CDPFK1_*DJPFK2_*J BPHIS3BP LGPM1_*LMPDC1_*M ARARS1211AR sga1)
|
This study; Prototrophic strain with native
and watermarked
single locus glycolysis (DG strain); Derived from IMX1748 |
| Strains Used As Starting
Point
or Intermediate in the Construction of the above Strains |
| CEN.PK113–7D |
MATaMAL2–8cSUC2
|
Control strain;46,47 Starting strain for all construction
work;13 Contains a SinLoG in Chr. IX, with
variable promoter and terminator length; Uracil auxotroph |
| IMX589 |
MATaura3–52his3–1leu2–3,112MAL2–8cSUC2glk1::Sphis5hxk1::KlLEU2tdh1tdh2gpm2gpm3eno1pyk2pdc5pdc6adh2adh5adh4sga1::(FBA1GHTPI1HPPGK1PQADH1QNPYK1NOTDH3OAENO2ABHXK2BCPGI1CDPFK1DJPFK2JKAmdSYMKLGPM1LMPDC1-SYNMF)pyk1pgi1tpi1tdh3pfk2::(pTEF-cas9-tCYC1 natNT1)pgk1gpm1fba1hxk2pfk1adh1pdc1eno2
|
|
| IMX1338 |
MATaura3–52his3–1leu2–3,112MAL2–8cSUC2hxk1::KlLEU2tdh1tdh2gpm2gpm3eno1pyk2pdc5pdc6adh2adh5adh4sga1::(FBA1GHTPI1HPPGK1PQADH1QNPYK1NOTDH3OAENO2ABHXK2BCPGI1CDPFK1DJPFK2JKAmdSYMKLGPM1LMPDC1-SYNMF)pyk1pgi1tpi1tdh3pfk2::(pTEF-cas9-tCYC1 natNT1)pgk1gpm1fba1hxk2pfk1adh1pdc1eno2glk1::Sphis5Δ::(pGAL1-ISceI-tCYC1)
|
This study; Derived from IMX589;
Contains a SinLoG in Chr.
IX; Uracil and histidine auxotroph |
| IMX1717 |
MATaura3–52his3–1leu2–3,112MAL2–8cSUC2hxk1::KlLEU2tdh1tdh2gpm2gpm3eno1pyk2pdc5pdc6adh2adh5adh4sga1::(FBA1GHTPI1HPPGK1PQADH1QNPYK1NOTDH3OAENO2ABHXK2BCPGI1CDPFK1DJPFK2JKAmdSYMKLGPM1LMPDC1-SYNMF)pgk1gpm1fba1hxk2pfk1adh1pdc1eno2glk1::Sphis5Δ::(pGAL1-I SceI-tCYC1)can1::(ARS418AHFBA1_*AH.HTPI1_*HPPGK1_*PQADH1_*QNPYK1_*NOTDH3_*OAENO2_*ABHXK2_*BCPGI1_*CDPFK1_*DJPFK2_*J.BPHIS3BP.LGPM1_*LMPDC1_*M.ARARS1211AR)
|
This study; Derived from IMX1338;
Strain with native (variable
prom and term length) and watermarked SinLoG in Chr. IX and V respectively;
Histidine auxotroph |
| IMX1747 |
MATaura3–52his3–1leu2–3,112MAL2–8cSUC2hxk1::KlLEU2tdh1tdh2gpm2gpm3eno1pyk2pdc5pdc6adh2adh5adh4 sga1::(FBA1GHTPI1HPPGK1PQADH1QNPYK1NOTDH3OAENO2ABHXK2BCPGI1CDPFK1DJPFK2JKAmdSYMKLGPM1LMPDC1-SYNMF)pyk1pgi1tpi1tdh3pfk2::(pTEF-cas9-tCYC1
natNT1)pgk1gpm1fba1hxk2pfk1adh1pdc1eno2glk1::Sphis5Δ::(pGAL1-I SceI-tCYC1)can1::(pGAL1-I-SceI-tCYC1)can1::(ARS418CAN1 AHFBA1AH HTPI1HPPGK1PQADH1QNPYK1NOTDH3OAENO2ABHXK2BCPGI1CDPFK1DJPFK2J BPHIS3BP LGPM1LMPDC1M ARARS1211AR CAN1)
|
This study; Derived from IMX1338; Strain
with native SinLoG
with variable prom and term length in Chr. IX and native SinLoG with
standardized prom and term length in Chr. V; Histidine auxotroph |
| IMX1748 |
MATaura3–52his3–1leu2–3,112MAL2–8cSUC2hxk1::KlLEU2tdh1tdh2gpm2gpm3eno1pyk2pdc5pdc6adh2adh5adh4pyk1pgi1tpi1tdh3pfk2::(pTEF-cas9-tCYC1 natNT1)pgk1gpm1fba1hxk2pfk1adh1pdc1eno2glk1::Sphis5Δ::(pGAL1-I-SceI-tCYC1)can1::(ARS418CAN1 AHFBA1AH HTPI1HPPGK1PQADH1QNPYK1NOTDH3OAENO2ABHXK2BCPGI1CDPFK1DJPFK2J BPHIS3BP LGPM1LMPDC1M ARARS1211AR CAN1)
|
This study; Derived from IMX1771; Strain
with watermarked SinLoG
in Chr. IX and native SinLoG with standardized prom and term length
in Chr. V; Uracil auxotroph |
