Skip to main content
. Author manuscript; available in PMC: 2020 Jun 23.
Published in final edited form as: ACS Appl Mater Interfaces. 2019 Mar 8;11(11):10472–10480. doi: 10.1021/acsami.8b20206

Figure 4. Co-delivery of anti-GFP sgRNA and Cas9 plasmid enables CRISPR-mediated gene knockout.

Figure 4.

(A) HEK-293T cells were transfected with R6,7,8_64 10 w/w nanoparticles encapsulating Cas9 DNA and sgRNA at the indicated nucleic acid molar ratios. N = 4. (B) Flow cytometry histograms of CRISPR- or siRNA-treated cells. CRISPR treatment produced a completely GFP-negative population (null) while siRNA treatment mainly resulted in a general population shift to lower GFP fluorescence (low). (C) Gene suppression kinetics of CRISPR and siRNA treated cells. N = 4.