Table 1.
Identification of Six New Dengue Virus Serotype 3 Specific Human Neutralizing Antigenic Sites CELL-HOST-MICROBE-D-19–01064R2
| A Name | Recombinant Virus Backbone | DENV3 5J7 Neut | DENV1 1F4 Neut | AA changes | Epitope Transplant | Neutralized by hmAb |
|---|---|---|---|---|---|---|
| DENV4/3 M16 | DENV4 Baric genotype I | +++ | - | 36 aa | 5J7 DENV3 | 5J7 |
| DENV3/1 EDI A | DENV3 Baric genotype III | - | +++ | 23 aa | 1F4 DENV1 |
66, 115, 144, 290, 419 |
| DENV3/1 EDI B | DENV3 Baric genotype III | - | +++ | 23 aa | 1F4 + 14c10 DENV1 | 66, 115, 144, 290, 419 |
| DENV3/1 EDI/III C | DENV3 Baric genotype III | +++ | +++ | 35 aa | 1F4 + 14c10 DENV1 | 115, 290, 419, 5J7 |
| DENV3/1 EDI/III D | DENV3 Baric genotype III | +++ | 37 aa | 1F4 + 14c10 DENV1 | 115, 290, 419 | |
| DENV1/3 EDI A | DENV1 Baric | - | - | 18 aa | EDI surface aa DENV3 | 236, 286, 297, 298, 354, 404, 406, 437, 443, 144 |
| DENV1/3 EDI B | DENV1 Baric | - | - | 22 aa | EDI all aa DENV3 | 236, 286, 297, 298, 354, 404, 406, 437, 443, 144 |
| B Name | Recombinant Virus Backbone | Domain Swap | AA changes | DENV-236 | DENV-297 | DENV-415 | DENV-115 | DENV-419 | DENV-66 |
|---|---|---|---|---|---|---|---|---|---|
| DENV3 G-III | DENV3 baric genotype III Sri Lanka | None | 0 | +++ | +++ | +++ | +++ | +++ | +++ |
| DENV3 G-IV | DENV3 baric genotype IV Puerto Rico | None | 0 | - | - | + | - | - | - |
| DENV3 G-IV with G-III EDI | DENV3 baric genotype IV Puerto Rico | EDI | 9 aa | +++ | +++ | + | - | - | - |
| DENV3 G-IV with G-III EDII | DENV3 baric genotype IV Puerto Rico | EDII | 9 aa | - | - | + | +++ | +++ | - |
| DENV3 G-IV with G-III EDIII | DENV3 baric genotype IV Puerto Rico | EDIII | 6 aa | - | - | +++ | + | - | +++ |
| DENV3 G-with G-EDI | DENV3 baric genotype III Sri Lanka | EDI | 8 aa | - | - | ++ | +++ | +++ | +++ |
| DENV3 G-with G-EDII | DENV3 baric genotype III Sri Lanka | EDII | 10 aa | +++ | +++ | ++ | +++ | ||
| DENV3 G-with G-EDIII | DENV3 baric genotype III Sri Lanka | EDIII | 6 aa | +++ | +++ | +++ | +++ | +++ | - |