KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| DENV-66 (hybridoma-produced Ig) | This manuscript | N/A |
| DENV-115 (hybridoma-produced Ig) | This manuscript | N/A |
| DENV-144 (hybridoma-produced Ig) | This manuscript | N/A |
| DENV-236 (hybridoma-produced Ig) | This manuscript | N/A |
| DENV-297 (hybridoma-produced Ig) | This manuscript | N/A |
| DENV-286 (hybridoma-produced Ig) | This manuscript | N/A |
| DENV-290 (hybridoma-produced Ig) | This manuscript | N/A |
| DENV-298 (hybridoma-produced Ig) | This manuscript | N/A |
| DENV-254 (hybridoma-produced Ig) | This manuscript | N/A |
| DENV-404 (hybridoma-produced Ig) | This manuscript | N/A |
| DENV-406 (hybridoma-produced Ig) | This manuscript | N/A |
| DENV-415 (hybridoma-produced Ig) | This manuscript | N/A |
| DENV-419 (hybridoma-produced Ig) | This manuscript | N/A |
| DENV-437 (hybridoma-produced Ig) | This manuscript | N/A |
| DENV-443 (hybridoma-produced Ig) | This manuscript | N/A |
| DENV-5J7 (plasmid-produced Ig) | Beltramello, Williams et al. 2010 | N/A |
| DENV-14c10 (plasmid-produced Ig) | Teoh, Kukkaro et al. 2012 | N/A |
| DENV-1F4 (hybridoma-produced Ig) | Fibiansah, Tan et al. 2014 | N/A |
| EDE1-C8 (plasmid-produced Ig) | Rouivinski, Guardado-Calvo et al. 2015 | N/A |
| EDE1-C10 (plasmid-produced Ig) | Rouivinski, Guardado-Calvo et al. 2015 | N/A |
| DENV-2D22 (hybridoma-produced Ig) | Smith et al., 2015 | N/A |
| Goat anti-Human IgG (Fc)-AP | Meridian Life Science, Inc. | Cat# W99008A |
| DENV-4G2(hybridoma-produced Ig) | ATCC® HB-112™ | D1–4G2–4-15 |
| DENV-2H2(hybridoma-produced Ig) | ATCC® HB-114™ | D3–2H2–9-21 |
| Bacterial and Virus Strains | ||
| DENV1 Thailand/16007/1964 | Buddhari et al., 2014, PMC4199527 | N/A |
| DENV1 Nauru/West Pac/1974 | Puri et al., 2000, DOI: 10.1023/A:1008160123754 | N/A |
| DENV1 N1265–04 | Andrade et al., 2017 | N/A |
| DENV1ic West Pac 74 | Gallichotte et al., 2017 | N/A |
| DENV2 Thailand/16681/1964 | Buddhari et al., 2014, PMC4199527 | N/A |
| DENV2 Thailand/S16803/1974 | Kelly et al., 2011, DOI 10.1007/s11262–011-0602-z | N/A |
| DENV2 N172–06 | Andrade et al., 2017 | N/A |
| DENV2ic 16803 | Gallichotte et al., 2015 | N/A |
| DENV3 Philippines/16562/964 | Buddhari et al., 2014, PMC4199527 | N/A |
| DENV3 Thailand/CH53489/1973 | Wahala et al., 2010 | N/A |
| DENV3 N2845–09 | Hadjilaou et al., 2015, doi:10.4049/jimmunol.1500918 | N/A |
| DENV3ic SriLanka 89 GIII | Messer et al., 2012 | N/A |
| DENV3 UNC3009, G-III | Messer et al., 2016 | N/A |
| DENV4 Indonesia/1036/1976 | Buddhari et al., 2014, PMC4199527 | N/A |
| DENV4 Columbia/TVP-376/1982 | Sukupolve-Petty et al., 2013 | N/A |
| DENV4 N703–99 | Hadjilaou et al., 2015, doi:10.4049/jimmunol.1500918 | N/A |
| DENV4ic SriLanka 92 | Widman, Young et al. 2017 | N/A |
| DENV3/4 M16 | Widman, Young et al. 2017 | N/A |
| DENV3/1 EDI A | Messer, Yount et al. 2016 | N/A |
| DENV3/1 EDI B | This manuscript | N/A |
| DENV3/1 EDI/III C | This manuscript | N/A |
| DENV3/1 EDI/III D | This manuscript | N/A |
| DENV1/3 EDI A | This manuscript | N/A |
| DENV1/3 EDI B | This manuscript | N/A |
| DENV3 Indonesia 1982, genotype I | Messer, Yount et al. 2012 | N/A |
| DENV3 Thailand 1995, genotype II | Messer, Yount et al. 2012 | N/A |
| DENV3 Cuba 2002, genotype III | Messer, Yount et al. 2012 | N/A |
| DENV3 Puerto Rico 1977, genotype IV | Messer, Yount et al. 2012 | N/A |
| DENV3 GIV with GIII EDI | This manuscript | N/A |
| DENV3 GIV with GIII EDII | This manuscript | N/A |
| DENV3 GIV with GIII EDIII | This manuscript | N/A |
| DENV3 GIII with GIV EDI | This manuscript | N/A |
| DENV3 GIII with GIV EDII | This manuscript | N/A |
| DENV3 GIII with GIV EDII | This manuscript | N/A |
| Biological Samples | ||
| PBMCs from DENV infection survivors | This manuscript | Nicaraguan Pediatric Dengue Cohort Study Donor ID #3243, #985, and #1791 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| 1-Step Ultra TMB-ELISA | Thermo Fisher Scientific | Cat# 34029 |
| Dulbecco’s Phosphate-Buffered Saline, 1X with calcium and magnesium | Corning Life Sciences | Cat# 21–030-CM |
| 50x HAT media supplement | Sigma-Aldrich | Cat# H0137 |
| Ouabain | Sigma-Aldrich | Cat# O3125 |
| HyClone insect cell culture medium | GE Healthcare Life Sciences | Cat# SH30280.03 |
| Fetal Bovine Serum, ultra-low IgG | Thermo Fisher Scientific | Cat# 16250078 |
| 100x Penicillin Streptomycin Glutamine | Thermo Fisher Scientific | Cat# 10378016 |
| ClonaCell-HY Medium E | Stem Cell Technologies | Cat# 03805 |
| ClonaCell-HY Medium A | Stem Cell Technologies | Cat# 03801 |
| DMEM, high glucose, GlutaMAX™ Supplement | Thermo Fisher Scientific | Cat# 10566–024 |
| Dulbecco’s modified Eagle’s/Ham’s F-12 50/50 Mix | Gibco | 10–092-CV |
| Opti-MEM I | Gibco | 31985–070 |
| GIBCO Hybridoma-SFM | Thermo Fisher Scientific | Cat# 12045076 |
| Recombinant DENV E protein | Kudlacek et al., 2018 | N/A |
| Recombinant DENV E protein stabilized dimers | Kudlacek et al., 2018 | N/A |
| CpG10103 (TCGTCGTTTTTCGGTCGTTTT) | Synthesized by Invitrogen | N/A |
| TrueBlue substrate | KPL | 0510–0050 |
| Cyclosporin A | Sigma-Aldrich | Cat# C1832 |
| Chk2 inhibitor | Sigma-Aldrich | Cat# C3742 |
| Non-fat dry milk | Bio Rad | Cat# 1706404 |
| Goat serum | Thermo Fisher Scientific | Cat# 16210072 |
| Critical Commercial Assays | ||
| Deposited Data | ||
| Experimental Models: Cell Lines | ||
| Mouse-human HMAA 2.5 myeloma cell line | Dr. Marshall Posner | N/A |
| DENV-66 hybridoma clone | This manuscript | N/A |
| DENV-115 hybridoma clone | This manuscript | N/A |
| DENV-144 hybridoma clone | This manuscript | N/A |
| DENV-236 hybridoma clone | This manuscript | N/A |
| DENV-297 hybridoma clone | This manuscript | N/A |
| DENV-298 hybridoma clone | This manuscript | N/A |
| DENV-286 hybridoma clone | This manuscript | N/A |
| DENV-290 hybridoma clone | This manuscript | N/A |
| DENV-254 hybridoma clone | This manuscript | N/A |
| DENV-404 hybridoma clone | This manuscript | N/A |
| DENV-406 hybridoma clone | This manuscript | N/A |
| DENV-415 hybridoma clone | This manuscript | N/A |
| DENV-419 hybridoma clone | This manuscript | N/A |
| DENV-437 hybridoma clone | This manuscript | N/A |
| DENV-443 hybridoma clone | This manuscript | N/A |
| C6/36 cells | ATCC | CRL-1660 |
| U937-DC-SIGN cells | ||
| Vero-81 cells | ATCC | CCL-81 |
| Experimental Models: Organisms/Strains | ||
| Mouse: AG129 strain | M. Aguet (Swiss Institute for Experimental Cancer Research, Epalinges, Switzerland) | N/A |
| Recombinant DNA | ||
| Primer: DENV1–4, D1: 5'-TCA ATA TGC TGA AAC GCGCGA GAA ACC G | Harris et al., 1998, PMID: 9705406 | N/A |
| Primer: DENV1–4, D2: 5’-TTGCACCAACAGTCAATGTCTTCAGGTTC | Harris et al., 1998, PMID: 9705406 | N/A |
| Primer: DENV1–4, TS1: 5'-CGT CTC AGT GAT CCG GGG G | Harris et al., 1998, PMID: 9705406 | N/A |
| Primer: DENV1–4, TS2: 5'-CGCCAC AAG GGC CAT GAA CAG | Harris et al., 1998, PMID: 9705406 | N/A |
| Primer: DENV1–4, TS3: 5'-TAA CAT CAT CAT GAG ACAGAG C | Harris et al., 1998, PMID: 9705406 | N/A |
| Primer: DENV1–4, DEN4: 5'-TGT TGT CTT AAA CAA GAG AGG TC | Harris et al., 1998, PMID: 9705406 | N/A |
| Primer: DENV UNC 3009 Forward: GGACTGGATACACGCACCCA | This manuscript | N/A |
| Primer: DENV UNC 3009 Reverse: CATGTCTCTACCTTCTCGACTTGTCT | This manuscript | N/A |
| DENV UNC 3009 Probe: ACCTGGATGTCGGCTGAAGGAGCTTG | This manuscript | N/A |
| TaqMan™ Rodent GAPDH Control Reagents | Applied Biosystems | Cat. 4308313 |
| Software and Algorithms | ||
| GraphPad Prism 7.2 | GraphPad Software, Inc. | https://www.graphpad.com |
| PyMOL | Schrödinger, LLC | https://www.pymol.org/ |
| Other | ||
| ÄKTA pure chromatography system | GE Healthcare Life Sciences | N/A |
| EL406 washer dispenser | BioTek | N/A |
| Biostack microplate stacker | BioTek | N/A |
| HiTrap Protein G High Performance | GE Healthcare Life Sciences | Cat# 17–0404-01 |
| Immunospot | CTL | N/A |