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. Author manuscript; available in PMC: 2021 Jan 5.
Published in final edited form as: Gene. 2019 Sep 5;722:144058. doi: 10.1016/j.gene.2019.144058

Figure 1:

Figure 1:

Effects of temperature changes on adherent MSCs viability and metabolic activity immediately after experimental treatment

This figure illustrates metabolic activity (A) and cell counts (B) immediately after 4 hour exposure to temperatures (4°C, 23°C, 37°C) in Lactated Ringers. The design of the experiment is shown (C). MTS assay was used to assess metabolic activity and Live/Dead stain for cell quantification. The data represent mean values from biological triplicates from two and three cell lines.

LR= Lactated Ringers, MTS = MTS assay; *p<.01, ** p<.0001, One-way ANOVA with Tukey’s post-hoc test, n=18(MTS, 4hrs), n=27(Live stain as part of the Live/Dead Cytotoxicity assay, 4hrs)