Effects of temperature changes on non-adherent MSCs in Lactated Ringers at 24 hours after experimental treatment
This figure shows metabolic activity (A) and cell counts (B) of the non-adherent MSCs exposed to 4 hour incubation in temperatures (4, 23, 37°C ) in Lactated Ringers following a 24 hour recovery period in standard culture media. The data represent mean values from biological triplicates of two cell lines.
LR= Lactated Ringers, MTS = MTS assay, Hoechst = Hoechst 33258 nuclear stain; *p<.05, ** p<.01, ***p<.001, One-way ANOVA with Tukey’s post-hoc test, n=6 (non-adherent)