Figure 6.

Effects of the i.t. delivering rIL-33 (rIL33) or the knockdown of IL-33 (IL33). (a) Effects of the i.t. delivering rIL-33 (100 ng, Supplementary Figure 5(a)) on the expression of miR547-5p in the DRG and SDH, which were normalized to that in naive rats (= 1, dashed line). (b) and (c) Examples of Western blots. Gels (from left to right) in (b) and (c) were respectively loaded with lysates prepared from the right L4 to L6 DRG (b) and SDH (c) of rats without any treatment (naive), at day 7 after the i.t. infusion of sh-NC (10 µl, Table 1), or at day 3 or 7 after the infusion of sh-IL-33 (10 µl, Table 1). (d) and (e) Summary data (mean ± SD) showing the relative changes in the expression of IL-33 and ST2 proteins in the DRG (d) and SDH (e) normalized with that in naive animals (= 1, dashed line). #p < 0.05, ##p < 0.01, ###p < 0.001 (Dunnett’s post hoc test in one-way ANOVA in comparison with that in naive rats). (f) and (g) Summary data (mean ± SD) showing effects of the IL-33 knockdown on the expression of miR547-5p in the DRG (f) and SDH (g), which were normalized to that in naive rats (= 1, dashed line). sh-IL33: IL-33 was knocked down; CCI/sh-IL33: the CCI was performed in rats on day 7 after the IL-33 knockdown (see Supplementary Figure 5(b)). ***p < 0.001, unpaired t test in comparison of the miR547-5p expression after the CCI versus that in naive rats. Values in brackets indicate the number of rats tested.

DRG: dorsal root ganglia; SDH: spinal dorsal horn; IL33: interleukin-33; ST2: suppressor of tumorigenicity 2; CCI: constriction nerve injury; NC: nonspecific negative control sequence.