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Virus-specific IgG1 and IgG2a subtype responses. Endpoint titers of IgG1 and IgG2a subtypes in serum samples of the rAd5-G-H group were detected with purified inactivated RABV and CDV by ELISA. Endpoint titers were calculated as the reciprocals of the highest serum dilution that gave an OD at 405 nm 2.1 times higher than the cutoff value. (A) RABV-specific and (B) CDV-specific IgG1 and IgG2a subtype responses.
