FIGURE 4.

Recruitment and/or activation of DCs in mice vaccinated with rAd5-G-H. BALB/c mice (n = 6) were immunized i.m. with 10⁸ GFU rAd5-G-H, rAd5-GFP or DMEM. The inguinal lymph nodes (LNs) were collected at 3, 6, and 9 days after vaccination. Single-cell suspensions were prepared and stained with antibodies of DC and then analyzed by flow cytometry. (A,B) The gating strategies for analyzing the DCs (A) and representative flow cytometric plots for measuring recruited and/or activated DCs (B) are shown. (C) Analyses for activated DCs (CD11c⁺ CD86⁺, CD11c⁺ MHCI⁺, CD11c⁺ MHCII⁺) from the LNs at 3, 6, and 9 days after vaccination are presented. Statistical analyses were tested by Student’s t-test in SPSS 22.0 software. *P < 0.05, **P < 0.01, and ***P < 0.001.